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CLCNKA Polyclonal antibody

CLCNKA Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14402-1-AP

Synonyms

chloride channel Ka, ClC K1, CLCK1, CLCNKA, hClC Ka

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLCNKA Polyclonal antibody (14402-1-AP)

    WB analysis using 14402-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14402-1-AP (CLCNKA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using CLCNKA Polyclonal antibody (14402-1-AP)

    WB analysis of human kidney using 14402-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 14402-1-AP (CLCNKA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using CLCNKA Polyclonal antibody (14402-1-AP)

    WB analysis of mouse pancreas using 14402-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14402-1-AP (CLCNKA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using CLCNKA Polyclonal antibody (14402-1-AP)

    IP experiment of HEK-293 using 14402-1-AP

    IP result of anti-CLCNKA (IP:14402-1-AP, 5ug; Detection:14402-1-AP 1:500) with HEK-293 cells lysate 880ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CLCNKA Polyclonal antibody (14402-1-AP)

    IHC staining of human kidney using 14402-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14402-1-AP (CLCNKA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CLCNKA Polyclonal antibody (14402-1-AP)

    IHC staining of human kidney using 14402-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14402-1-AP (CLCNKA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CLCNKA Polyclonal antibody (14402-1-AP)

    IHC staining of human placenta using 14402-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CLCNKA Polyclonal antibody (14402-1-AP)

    IHC staining of human placenta using 14402-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CLCNKA Polyclonal antibody (14402-1-AP)

    IHC staining of human testis using 14402-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CLCNKA Polyclonal antibody (14402-1-AP)

    IHC staining of human testis using 14402-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CLCNKA Polyclonal antibody (14402-1-AP)

    IHC staining of human skin using 14402-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CLCNKA Polyclonal antibody (14402-1-AP)

    IHC staining of human skin using 14402-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CLCNKA Polyclonal antibody (14402-1-AP)

    IHC staining of human spleen using 14402-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CLCNKA Polyclonal antibody (14402-1-AP)

    IHC staining of human spleen using 14402-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using CLCNKA Polyclonal antibody (14402-1-AP)

    IHC staining of human lung using 14402-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using CLCNKA Polyclonal antibody (14402-1-AP)

    IHC staining of human lung using 14402-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using CLCNKA Polyclonal antibody (14402-1-AP)

    IHC staining of human ovary using 14402-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using CLCNKA Polyclonal antibody (14402-1-AP)

    IHC staining of human ovary using 14402-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, human kidney tissue, mouse pancreas tissue, mouse kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human spleen tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14402-1-AP targets CLCNKA in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLCNKA fusion protein Ag5647
Full Name chloride channel Ka
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC048282
Gene Symbol CLCNKA
Gene ID (NCBI) 1187
RRIDAB_2079857
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CLCNKA antibody 14402-1-APDownload protocol
IHC protocol for CLCNKA antibody 14402-1-APDownload protocol
IP protocol for CLCNKA antibody 14402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols