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Dectin-1 Polyclonal antibody

Dectin-1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22809-1-AP

Synonyms

Beta glucan receptor, BGR, CD369, CLEC7A, CLECSF12, DC associated C type lectin 1, Dectin 1, DECTIN1, Dectin-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Dectin-1 Polyclonal antibody (22809-1-AP)

    WB analysis using 22809-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22809-1-AP (Dectin-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHL-60 cells, THP-1 cells, U-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22809-1-AP targets Dectin-1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Dectin-1 fusion protein Ag18924
Full Name C-type lectin domain family 7, member A
Calculated Molecular Weight 247 aa, 28 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC013385
Gene Symbol CLEC7A
Gene ID (NCBI) 64581
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dectin-1, also known as CLEC7A or CD369, is a type II membrane receptor consisting of an extracellular C-terminal C-type lectin domain, a short stalk region, a single transmembrane domain and a short N-terminal cytoplasmic tail, which contains an immunoreceptor tyrosine-based activation motif (PMID: 10779524; 21612412). Dectin-1 is expressed on monocytes, macrophages, neutrophils, dendritic cells and a subpopulation of T cells (PMID: 12244185). Dectin-1 plays a role in the innate immune response. It functions as a pattern-recognition receptor and recognizes a variety of β-glucans from fungi, plants and bacteria. Dectin-1 may also participate in orchestrating the adaptive immune response (PMID: 21612412). The apparent molecular weight is larger than the calculated molecular weight due to glycosylation (PMID: 10779524).

Protocols

Product Specific Protocols
WB protocol for Dectin-1 antibody 22809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols