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  • Featured Product
  • KD/KO Validated

CLIP-115/CLIP2 Monoclonal antibody

CLIP-115/CLIP2 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2E6B6

Cat no : 67776-1-Ig

Synonyms

CLIP, CLIP 115, CLIP-115/CLIP2, CLIP2, CYLN2, Cytoplasmic linker protein 115, Cytoplasmic linker protein 2, KIAA0291, WBSCR3, WBSCR4, WSCR3, WSCR4

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse cerebellum tissue using CLIP-115/CLIP2 Monoclonal antibody (67776-1-Ig)

    WB analysis of mouse cerebellum using 67776-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using CLIP-115/CLIP2 Monoclonal antibody (67776-1-Ig)

    WB analysis of A549 using 67776-1-Ig

    WB result of CLIP-115/CLIP2 antibody (67776-1-Ig; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIP-115/CLIP2 transfected A549 cells.

  • Western Blot (WB) analysis of rabbit brain tissue using CLIP-115/CLIP2 Monoclonal antibody (67776-1-Ig)

    WB analysis of rabbit brain using 67776-1-Ig

    rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using CLIP-115/CLIP2 Monoclonal antibody (67776-1-Ig)

    WB analysis of pig brain using 67776-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using CLIP-115/CLIP2 Monoclonal antibody (67776-1-Ig)

    WB analysis of rat brain using 67776-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CLIP-115/CLIP2 Monoclonal antibody (67776-1-Ig)

    WB analysis of mouse brain using 67776-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig cerebellum tissue using CLIP-115/CLIP2 Monoclonal antibody (67776-1-Ig)

    WB analysis of pig cerebellum using 67776-1-Ig

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat cerebellum tissue using CLIP-115/CLIP2 Monoclonal antibody (67776-1-Ig)

    WB analysis of rat cerebellum using 67776-1-Ig

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CLIP-115/CLIP2 Monoclonal antibody (67776-1-Ig)

    IF Staining of A549 using 67776-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CLIP-115/CLIP2 antibody (67776-1-Ig, Clone: 2E6B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inmouse cerebellum tissue, rabbit brain tissue, pig brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rat cerebellum tissue, A549 cells
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67776-1-Ig targets CLIP-115/CLIP2 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CLIP-115/CLIP2 fusion protein Ag18902
Full Name CAP-GLY domain containing linker protein 2
Calculated Molecular Weight 1011 aa, 112 kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberBC150332
Gene Symbol CLIP2
Gene ID (NCBI) 7461
RRIDAB_2918541
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CILP-2/CLIP-115 is a cytoplasmic linker protein (CLIP) that links membranous organelles to microtubules. CLIP-115 is enriched in the axonal growth cone and it enables neuronal polarization by controlling the stabilization of microtubules. Mutation of CLIP-115 has been associated with Williams-Beuren syndrome.

Protocols

Product Specific Protocols
WB protocol for CLIP-115/CLIP2 antibody 67776-1-IgDownload protocol
IF protocol for CLIP-115/CLIP2 antibody 67776-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols