• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CLK1 Polyclonal antibody

CLK1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 20439-1-AP

Synonyms

CDC like kinase 1, CLK, CLK/STY, CLK1, STY

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CLK1 Polyclonal antibody (20439-1-AP)

    WB analysis using 20439-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CLK1 Polyclonal antibody (20439-1-AP)

    WB analysis of HepG2 using 20439-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HeLa cells using CLK1 Polyclonal antibody (20439-1-AP)

    WB analysis of HeLa using 20439-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using CLK1 Polyclonal antibody (20439-1-AP)

    WB analysis of COLO 320 using 20439-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using CLK1 Polyclonal antibody (20439-1-AP)

    IP experiment of COLO 320 using 20439-1-AP

    IP result of anti-CLK1 (IP:20439-1-AP, 4ug; Detection:20439-1-AP 1:300) with COLO 320 cells lysate 2000ug.

Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, HepG2 cells
Positive IP detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20439-1-AP targets CLK1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLK1 fusion protein Ag14214
Full Name CDC-like kinase 1
Calculated Molecular Weight 484 aa, 57 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC031549
Gene Symbol CLK1
Gene ID (NCBI) 1195
RRIDAB_2878686
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CLK1 antibody 20439-1-APDownload protocol
IP protocol for CLK1 antibody 20439-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols