Claspin Polyclonal antibody

Claspin Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 23206-1-AP

Synonyms

CLASPIN, CLSPN, hClaspin



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 23206-1-AP is Claspin Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Claspin fusion protein Ag19637
Full Name claspin homolog (Xenopus laevis)
Calculated Molecular Weight 1339 aa, 151 kDa
Observed Molecular Weight 240 kDa
GenBank Accession NumberBC137279
Gene Symbol CLSPN
Gene ID (NCBI) 63967
RRIDAB_2879232
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLSPN, also named as Claspin, is a 1339 amino acid protein, which belongs to the claspin family. CLSPN localizes in the nucleus and is highly expressed at S/G2 phases of the cell cycle. CLSPN is required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. CLSPN plays a potentially critical role in replication checkpoint control in cells and mediating phosphorylation and activation of checkpoint protein kinases. The calculated molecular weight of CLSPN is 151 kDa, but the modified protein is about 220-240 kDa.

Protocols

Product Specific Protocols
WB protocol for Claspin antibody 23206-1-APDownload protocol
IF protocol for Claspin antibody 23206-1-APDownload protocol
IP protocol for Claspin antibody 23206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Mol Life Sci

HAS3-induced extracellular vesicles from melanoma cells stimulate IHH mediated c-Myc upregulation via the hedgehog signaling pathway in target cells.

Authors - Uma Thanigai Arasu
humanWB

Int J Oncol

Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells.

Authors - Chao Wang