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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CLTC Monoclonal antibody

CLTC Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B3D7

Cat no : CL594-66487

Synonyms

CHC, CHC17, Clathrin heavy chain 1, clathrin, heavy chain (Hc), CLH 17, CLH17, CLTC, CLTCL2, Hc, KIAA0034

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated CLTC Monoclonal antibody (CL594-66487)

    IF Staining of HepG2 using CL594-66487

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66487 (CLTC antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66487 targets CLTC in IF applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity Human, Pig, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CLTC fusion protein Ag25040
Full Name clathrin, heavy chain (Hc)
Calculated Molecular Weight 192 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC054489
Gene Symbol CLTC
Gene ID (NCBI) 1213
RRIDAB_2883592
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (CLTC) (160-190 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The heavy chain provides the structural backbone of the clathrin lattice. (PMID: 9133677)

Protocols

Product Specific Protocols
IF protocol for CL594 CLTC antibody CL594-66487Download protocol
Standard Protocols
Click here to view our Standard Protocols