CMTM8 Polyclonal antibody

CMTM8 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15039-1-AP

Synonyms

CKLFSF8, CKLFSF8 V2, CMTM8



Tested Applications

Positive WB detected inmouse liver tissue, mouse pancreas tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue, human pancreas tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15039-1-AP targets CMTM8 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CMTM8 fusion protein Ag6776
Full Name CKLF-like MARVEL transmembrane domain containing 8
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC041390
Gene Symbol CMTM8
Gene ID (NCBI) 152189
RRIDAB_10949191
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CMTM8, also known as CKLFSF8, is a member of the chemokine-like factor superfamily (CKLFSF). Proteins of this family share similarity with both the chemokine and the transmembrane 4 superfamilies. CMTM8 is an attenuator of EGF-induced signaling (PMID: 16263120). Overexpression of CMTM8 accelerates ligand-induced clearance of EGFR from the cell surface and induces apoptosis via caspase-dependent and -independent pathways (PMID: 17149703). An alternative splice form of CMTM8, CMTM8-v2, arises from a deletion of exon 2. CMTM8-v2 maintains the ability to induce apoptosis (PMID: 17681841).

Protocols

Product Specific Protocols
WB protocol for CMTM8 antibody 15039-1-APDownload protocol
IHC protocol for CMTM8 antibody 15039-1-APDownload protocol
IP protocol for CMTM8 antibody 15039-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

DNA Cell Biol

Downregulated CMTM8 Correlates with Poor Prognosis in Gastric Cancer Patients.

Authors - Meng Yan
humanIHC

BMC Gastroenterol

Identification and validation of EMT-immune-related prognostic biomarkers CDKN2A, CMTM8 and ILK in colon cancer.

Authors - Ning Kang
humanWB

Oncol Rep

CMTM8 inhibits the carcinogenesis and progression of bladder cancer.

Authors - Denghui Gao
humanWB

Bioengineered

MicroRNA-582-5p promotes triple-negative breast cancer invasion and metastasis by antagonizing CMTM8

Authors - Xue Zeng
IHC

Cancers (Basel)

The MAL Family of Proteins: Normal Function, Expression in Cancer, and Potential Use as Cancer Biomarkers

Authors - Leticia Labat-de-Hoz