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  • Featured Product
  • KD/KO Validated

CNKSR1 Polyclonal antibody

CNKSR1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10885-1-AP

Synonyms

CNK, CNK homolog protein 1, CNK1, CNKSR1, Connector enhancer of KSR 1, Connector enhancer of KSR like, hCNK1, KSR

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using CNKSR1 Polyclonal antibody (10885-1-AP)

    WB analysis of HT-29 using 10885-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 10885-1-AP (CNKSR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CNKSR1 Polyclonal antibody (10885-1-AP)

    WB analysis of human brain using 10885-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10885-1-AP (CNKSR1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HT-29 cells using CNKSR1 Polyclonal antibody (10885-1-AP)

    IP experiment of HT-29 using 10885-1-AP

    IP result of anti-CNKSR1 (IP:10885-1-AP, 4ug; Detection:10885-1-AP 1:1000) with HT-29 cells lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CNKSR1 Polyclonal antibody (10885-1-AP)

    IHC staining of human pancreas cancer using 10885-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10885-1-AP (CNKSR1 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CNKSR1 Polyclonal antibody (10885-1-AP)

    IHC staining of human pancreas cancer using 10885-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10885-1-AP (CNKSR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHT-29 cells, human brain tissue
Positive IP detected inHT-29 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10885-1-AP targets CNKSR1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNKSR1 fusion protein Ag1333
Full Name connector enhancer of kinase suppressor of Ras 1
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC011604
Gene Symbol CNKSR1
Gene ID (NCBI) 10256
RRIDAB_2276387
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CNKSR1 is a protein containing several motifs involved in protein-protein interaction, including PDZ, PH (Pleckstrin homology), and SAM (sterile alpha motif) domains. The encoded protein acts as a scaffold component for receptor tyrosine kinase signaling and may mediate crosstalk between different signaling pathways. CNKSR1 is required in multiple receptor tyrosine kinase pathways where it appears to be a tyrosine phosphorylation targe.

Protocols

Product Specific Protocols
WB protocol for CNKSR1 antibody 10885-1-APDownload protocol
IHC protocol for CNKSR1 antibody 10885-1-APDownload protocol
IP protocol for CNKSR1 antibody 10885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Life Sci Alliance

CNKSR1 serves as a scaffold to activate an EGFR phosphatase via exclusive interaction with RhoB-GTP.

Authors - Kanako Nishiyama
  • KD Validated
View Article
humanIHC

Cancer Rep (Hoboken)

Identification of hub genes in bladder cancer based on weighted gene co-expression network analysis from TCGA database.

Authors - Lei Wang
View Article