• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CNPY4 Polyclonal antibody

CNPY4 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 22086-1-AP

Synonyms

canopy 4 homolog (zebrafish), CNPY4, PRAT4B, Protein canopy homolog 4, UNQ1909/PRO4354

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using CNPY4 Polyclonal antibody (22086-1-AP)

    WB analysis of HeLa using 22086-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22086-1-AP (CNPY4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CNPY4 Polyclonal antibody (22086-1-AP)

    WB analysis of HeLa using 22086-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22086-1-AP (CNPY4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CNPY4 Polyclonal antibody (22086-1-AP)

    IF Staining of HeLa using 22086-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22086-1-AP (CNPY4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22086-1-AP is CNPY4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNPY4 fusion protein Ag17285
Full Name canopy 4 homolog (zebrafish)
Calculated Molecular Weight 248 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC032339
Gene Symbol CNPY4
Gene ID (NCBI) 245812
RRIDAB_2878990
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CNPY4 antibody 22086-1-APDownload protocol
IF protocol for CNPY4 antibody 22086-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols