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COG7 Polyclonal antibody

COG7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13552-1-AP

Synonyms

CDG2E, COG complex subunit 7, COG7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COG7 Polyclonal antibody (13552-1-AP)

    WB analysis using 13552-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13552-1-AP (COG7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using COG7 Polyclonal antibody (13552-1-AP)

    WB analysis of Y79 using 13552-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 13552-1-AP (COG7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using COG7 Polyclonal antibody (13552-1-AP)

    IHC staining of human cervical cancer using 13552-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13552-1-AP (COG7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using COG7 Polyclonal antibody (13552-1-AP)

    IHC staining of human cervical cancer using 13552-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13552-1-AP (COG7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, Y79 cells, HeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13552-1-AP targets COG7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COG7 fusion protein Ag4335
Full Name component of oligomeric golgi complex 7
Calculated Molecular Weight 770 aa, 86 kDa
Observed Molecular Weight 86 kDa
GenBank Accession NumberBC037563
Gene Symbol COG7
Gene ID (NCBI) 91949
RRIDAB_2229608
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for COG7 antibody 13552-1-APDownload protocol
IHC protocol for COG7 antibody 13552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Boyan (Verified Customer) (09-28-2020)

This antibody recognised a clean band at the expected molecular weight.

  • Applications: Western Blot