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COQ10A Polyclonal antibody

COQ10A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17812-1-AP

Synonyms

COQ10A

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human heart tissue using COQ10A Polyclonal antibody (17812-1-AP)

    WB analysis of human heart using 17812-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 17812-1-AP (COQ10A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using COQ10A Polyclonal antibody (17812-1-AP)

    WB analysis of mouse skeletal muscle using 17812-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17812-1-AP (COQ10A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using COQ10A Polyclonal antibody (17812-1-AP)

    IHC staining of human liver using 17812-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17812-1-AP (COQ10A antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using COQ10A Polyclonal antibody (17812-1-AP)

    IHC staining of human liver using 17812-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17812-1-AP (COQ10A antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COQ10A Polyclonal antibody (17812-1-AP)

    IF Staining of HepG2 using 17812-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17812-1-AP (COQ10A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman heart tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17812-1-AP targets COQ10A in WB, IHC, IF, ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COQ10A fusion protein Ag12126
Full Name coenzyme Q10 homolog A (S. cerevisiae)
Calculated Molecular Weight 247 aa, 28 kDa
Observed Molecular Weight 20-23 kDa
GenBank Accession NumberBC073923
Gene Symbol COQ10A
Gene ID (NCBI) 93058
RRIDAB_2081926
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for COQ10A antibody 17812-1-APDownload protocol
IHC protocol for COQ10A antibody 17812-1-APDownload protocol
IF protocol for COQ10A antibody 17812-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Front Pharmacol

Propofol Augments Paclitaxel-Induced Cervical Cancer Cell Ferroptosis In Vitro.

Authors - Meng-Yun Zhao
View Article
humanWB

Mol Med Rep

Depletion of ubiA prenyltransferase domain containing 1 expression promotes angiotensin II‑induced hypertrophic response in AC16 human myocardial cells via modulating the expression levels of coenzyme Q10 and endothelial nitric oxide synthase.

Authors - Bingju Yan
View Article
ratWB

Mol Med Rep

UBIAD1 expression is associated with cardiac hypertrophy in spontaneously hypertensive rats.

Authors - Bingju Yan
View Article
humanWB

J Lipid Res

Human COQ10A and COQ10B are distinct lipid-binding START domain proteins required for coenzyme Q function.

Authors - Hui S Tsui
View Article