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CORO2B Polyclonal antibody

CORO2B Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 13802-1-AP

Synonyms

Clipin C, CLIPINC, CORO2B, Coronin 2B, Coronin 5, Coronin like protein C, KIAA0925, Protein FC96

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CORO2B Polyclonal antibody (13802-1-AP)

    WB analysis of Jurkat using 13802-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13802-1-AP (CORO2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CORO2B Polyclonal antibody (13802-1-AP)

    WB analysis of mouse brain using 13802-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13802-1-AP (CORO2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CORO2B Polyclonal antibody (13802-1-AP)

    IHC staining of human ovary tumor using 13802-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13802-1-AP (CORO2B antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CORO2B Polyclonal antibody (13802-1-AP)

    IHC staining of human ovary tumor using 13802-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13802-1-AP (CORO2B antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CORO2B Polyclonal antibody (13802-1-AP)

    IHC staining of human ovary tumor using 13802-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13802-1-AP (CORO2B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CORO2B Polyclonal antibody (13802-1-AP)

    IHC staining of human ovary tumor using 13802-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13802-1-AP (CORO2B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, mouse brain tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13802-1-AP targets CORO2B in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CORO2B fusion protein Ag4793
Full Name coronin, actin binding protein, 2B
Calculated Molecular Weight 475 aa, 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC026335
Gene Symbol CORO2B
Gene ID (NCBI) 10391
RRIDAB_2087042
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CORO2B antibody 13802-1-APDownload protocol
IHC protocol for CORO2B antibody 13802-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols