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CPLX2 Polyclonal antibody

CPLX2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20249-1-AP

Synonyms

921 L, complexin 2, Complexin II, CPLX2, CPX 2, CPX II, CPX2, Hfb1, Synaphin 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using CPLX2 Polyclonal antibody (20249-1-AP)

    WB analysis of mouse brain using 20249-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20249-1-AP (CPLX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using CPLX2 Polyclonal antibody (20249-1-AP)

    IHC staining of human brain using 20249-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 20249-1-AP (CPLX2 Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20249-1-AP targets CPLX2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name complexin 2
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberNM_006650
Gene Symbol CPLX2
Gene ID (NCBI) 10814
RRIDAB_11232598
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom. This antibody is expected to specifically recogize CPLX2 but not other complexins.

Protocols

Product Specific Protocols
WB protocol for CPLX2 antibody 20249-1-APDownload protocol
IHC protocol for CPLX2 antibody 20249-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols