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- KD/KO Validated
CPT1C Monoclonal antibody
CPT1C Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
5D11B5
Cat no : 66072-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Human brain, human brain tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 66072-1-Ig is CPT1C Fusion Protein expressed in E. coli.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CPT1C fusion protein Ag17963 |
| Full Name | carnitine palmitoyltransferase 1C |
| Calculated Molecular Weight | 792 aa, 90 kDa |
| Observed Molecular Weight | 82 kDa |
| GenBank Accession Number | BC029104 |
| Gene Symbol | CPT1C |
| Gene ID (NCBI) | 126129 |
| RRID | AB_11042325 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CPT1C, also named as CATL1, CPTI-B and B-CPTI, belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1C is an active forms of related brain-type carnitine palmitoyltransferase I. CPT1C may be a regulated target of malonyl-CoA that relays the "malonyl-CoA signal" in hypothalamic neurons that express the orexigenic and anorexigenic neuropeptides that regulate food intake and peripheral energy expenditure. (PMID: 17018521 ) This antibody can bind the close sequences genes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CPT1C antibody 66072-1-Ig | Download protocol |
| IHC protocol for CPT1C antibody 66072-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hepatology Discovery of a CPS1-deficient HCC subtype with therapeutic potential via integrative genomic and experimental analysis.
| ||
Cancer Metab MicroRNA-377-3p inhibits hepatocellular carcinoma growth and metastasis through negative regulation of CPT1C-mediated fatty acid oxidation. | ||
Epigenetics Differential regulatory roles of microRNAs during intramuscular adipogenesis in Chinese Guizhou Congjiang Xiang pigs. | ||
J Biochem Mol Toxicol Polyphyllin I improves myocardial damage in coronary artery disease via modulating lipid metabolism and myocardial apoptosis | ||
J Gastrointest Oncol Enhanced fatty acid oxidation mediated by CPT1C promotes gastric cancer progression.
| ||
Int J Mol Sci Cpt1c Downregulation Causes Plasma Membrane Remodelling and Anthracycline Resistance in Breast Cancer
|
