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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CRABP2 Monoclonal antibody

CRABP2 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A5F3

Cat no : CL594-66468

Synonyms

CRABP II, CRABP2, RBP6

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human skin cancer tissue using CoraLite®594-conjugated CRABP2 Monoclonal antibody (CL594-66468)

    IF Staining of human skin cancer using CL594-66468

    Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CoraLite®594-conjugated CRABP2 antibody (CL594-66468, Clone: 1A5F3 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite®594-conjugated CRABP2 Monoclonal antibody (CL594-66468)

    FC experiment of MCF-7 using CL594-66468

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CRABP2 (CL594-66468, Clone:1A5F3) (red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inhuman skin cancer tissue
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66468 targets CRABP2 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CRABP2 fusion protein Ag0309
Full Name cellular retinoic acid binding protein 2
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC001109
Gene Symbol CRABP2
Gene ID (NCBI) 1382
RRIDAB_2919998
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

Protocols

Product Specific Protocols
IF protocol for CL594 CRABP2 antibody CL594-66468Download protocol
FC protocol for CL594 CRABP2 antibody CL594-66468Download protocol
Standard Protocols
Click here to view our Standard Protocols