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  • KD/KO Validated

CRBN Polyclonal antibody

CRBN Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 28494-1-AP

Synonyms

cereblon, CRBN, DKFZp781K0715, MRT2A, Protein cereblon



Tested Applications

Positive WB detected inCOLO 320 cells, mouse liver tissue, 3T3-L1 cells, THP-1 cells, U-251 cells, C6 cells
Positive IHC detected inmouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28494-1-AP targets CRBN in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, rat samples.

Tested Reactivity Human, Mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRBN fusion protein Ag29353
Full Name cereblon
Calculated Molecular Weight 442 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC017419
Gene Symbol CRBN
Gene ID (NCBI) 51185
RRIDAB_2881155
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRBN plays an important role in assembly and surface expression of functional BK(Ca) channels. The mutation of human cereblon gene (CRBN) is related with mild mental retardation, and causes ARNSID (nonsyndromal intellectual disability) by distubing the developmental regulation of BK(Ca) in brain regions that are critical for memory and learning. CRBN is also identified as important thalidomide-binding protein for limb outgrowth in zebrafish and chicks. It can be detected a band above 51 kDa (maybe 55 kDa) in HT22 cells(PMID: 21232561).

Protocols

Product Specific Protocols
WB protocol for CRBN antibody 28494-1-APDownload protocol
IHC protocol for CRBN antibody 28494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Leukemia

Phase 2 study of oral thalidomide-cyclophosphamide-dexamethasone for recurrent/refractory adult Langerhans cell histiocytosis.

Authors - Ji-Nuo Wang
humanWB,IF,IP

Hum Mol Genet

Cereblon suppresses the formation of pathogenic protein aggregates in a p62-dependent manner.

Authors - Liang Zhou
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Angie (Verified Customer) (07-06-2023)

The antibody detected a band around 50 kD at dilution of 1:6000 incubated at room temperature for 1 hour followed by secondary antibody donkey-anti-rabbit (Alexa Fluor 800) at 1:20000 dilution, incubated 1 hour at room temperature.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:6000
  • Cell Tissue Type: HEK293, A549, H460, HeLa, MCF7
CRBN Antibody Western Blot validation (1:6000 dilution) in HEK293, A549, H460, HeLa, MCF7 (Cat no:28494-1-AP)