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  • KD/KO Validated

CRBN Monoclonal antibody

CRBN Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A9D11

Cat no : 66336-1-Ig

Synonyms

cereblon, CRBN, MRT2A, Protein cereblon



Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

66336-1-Ig targets CRBN in IHC, ELISA applications and shows reactivity with human, pig, mouse samples.

Tested Reactivity human, pig, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CRBN fusion protein Ag17166
Full Name cereblon
Calculated Molecular Weight 442 aa, 51 kDa
Observed Molecular Weight 51-60 kDa
GenBank Accession NumberBC017419
Gene Symbol CRBN
Gene ID (NCBI) 51185
RRIDAB_2881716
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRBN plays an important role in assembly and surface expression of functional BK(Ca) channels. The mutation of human cereblon gene (CRBN) is related with mild mental retardation, and causes ARNSID (nonsyndromal intellectual disability) by distubing the developmental regulation of BK(Ca) in brain regions that are critical for memory and learning. CRBN is also identified as important thalidomide-binding protein for limb outgrowth in zebrafish and chicks. It can be detected a band above 51 kDa (maybe 55 kDa) in HT22 cells(PMID: 21232561).

Publications

SpeciesApplicationTitle
humanIHC

Front Oncol

Prediction Model for Cereblon Expression in Bone Marrow Plasma Cells Based on Blood Markers in Multiple Myeloma Patients.

Authors - Byung-Hyun Lee

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

S (Verified Customer) (11-22-2021)

I couldn't get this antibody to work. In my blots (tried 2-3 times on HEK293T), there are so many bands both in Nu and Cy factions. Hard to tell which band is for CRBN.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T, HCT116
CRBN Antibody Western Blot validation (1:1000 dilution) in HEK293T, HCT116 (Cat no:66336-1-Ig)