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CRELD2 Polyclonal antibody

CRELD2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 27017-1-AP

Synonyms

CRELD2

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using CRELD2 Polyclonal antibody (27017-1-AP)

    WB analysis of human placenta using 27017-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 27017-1-AP (CRELD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using CRELD2 Polyclonal antibody (27017-1-AP)

    WB analysis of SH-SY5Y using 27017-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 27017-1-AP (CRELD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using CRELD2 Polyclonal antibody (27017-1-AP)

    WB analysis of U2OS using 27017-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 27017-1-AP (CRELD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using CRELD2 Polyclonal antibody (27017-1-AP)

    WB analysis of human placenta using 27017-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 27017-1-AP (CRELD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CRELD2 Polyclonal antibody (27017-1-AP)

    IHC staining of human placenta using 27017-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27017-1-AP (CRELD2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CRELD2 Polyclonal antibody (27017-1-AP)

    IHC staining of human placenta using 27017-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27017-1-AP (CRELD2 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CRELD2 Polyclonal antibody (27017-1-AP)

    IHC staining of human pancreas using 27017-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 27017-1-AP (CRELD2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CRELD2 Polyclonal antibody (27017-1-AP)

    IHC staining of human pancreas using 27017-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 27017-1-AP (CRELD2 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inhuman placenta tissue, SH-SY5Y cells, U2OS cells
Positive IHC detected inhuman placenta tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27017-1-AP is CRELD2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRELD2 fusion protein Ag25553
Full Name cysteine-rich with EGF-like domains 2
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 30-45 kDa
GenBank Accession NumberBC050675
Gene Symbol CRELD2
Gene ID (NCBI) 79174
RRIDAB_2880723
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CRELD2 antibody 27017-1-APDownload protocol
IHC protocol for CRELD2 antibody 27017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols