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CRYZL1 Polyclonal antibody

CRYZL1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16894-1-AP

Synonyms

4P11, CRYZL1, Protein 4P11, QOH 1, Zeta crystallin homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of COS-7 cells using CRYZL1 Polyclonal antibody (16894-1-AP)

    WB analysis of COS-7 using 16894-1-AP

    COS-7 cells were subjected to SDS PAGE followed by western blot with 16894-1-AP (CRYZL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CRYZL1 Polyclonal antibody (16894-1-AP)

    WB analysis of MCF-7 using 16894-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16894-1-AP (CRYZL1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of COS-7 cells using CRYZL1 Polyclonal antibody (16894-1-AP)

    WB analysis of COS-7 using 16894-1-AP

    COS-7 cells were subjected to SDS PAGE followed by western blot with 16894-1-AP (CRYZL1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CRYZL1 Polyclonal antibody (16894-1-AP)

    IHC staining of human ovary tumor using 16894-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16894-1-AP (CRYZL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CRYZL1 Polyclonal antibody (16894-1-AP)

    IHC staining of human ovary tumor using 16894-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16894-1-AP (CRYZL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inCOS-7 cells, MCF-7 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16894-1-AP targets CRYZL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRYZL1 fusion protein Ag10419
Full Name crystallin, zeta (quinone reductase)-like 1
Calculated Molecular Weight 349 aa, 39 kDa
Observed Molecular Weight 39-45 kDa
GenBank Accession NumberBC013155
Gene Symbol CRYZL1
Gene ID (NCBI) 9946
RRIDAB_2878329
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CRYZL1 antibody 16894-1-APDownload protocol
IHC protocol for CRYZL1 antibody 16894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols