CSNK2A2 Polyclonal antibody

CSNK2A2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10606-1-AP

Synonyms

Casein kinase II subunit alpha', CK II alpha', CK2A2, CSNK2A1, CSNK2A2



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, HT-1080 cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10606-1-AP targets CSNK2A2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSNK2A2 fusion protein Ag0877
Full Name casein kinase 2, alpha prime polypeptide
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC008812
Gene Symbol CSNK2A2
Gene ID (NCBI) 1459
RRIDAB_2292447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSNK2A2(Casein kinase II subunit alpha') is also named as CK2A3, CK2A2, CSNK2A1, CK2 and belongs to the protein kinase superfamily. It can function as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage during mitosis. It also can protect against ionizing radiation-induced apoptosis partly by phosphorylating and activating SIRT1. This protein has molecular weights between 35 kDa and 44 kDa(PMID:8622692).

Protocols

Product Specific Protocols
WB protocol for CSNK2A2 antibody 10606-1-APDownload protocol
IF protocol for CSNK2A2 antibody 10606-1-APDownload protocol
IP protocol for CSNK2A2 antibody 10606-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Acta Neuropathol Commun

CK2 alpha prime and alpha-synuclein pathogenic functional interaction mediates synaptic dysregulation in huntington's disease.

Authors - Dahyun Yu
mouseWB

Biochem Biophys Res Commun

β-catenin stimulates Tcf7l1 degradation through recruitment of casein kinase 2 in mouse embryonic stem cells.

Authors - Yan Zhang
mouseWB

Cell Rep

Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's disease

Authors - Rachel H Mansky
  • KD Validated