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CST8 Polyclonal antibody

CST8 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 18183-1-AP

Synonyms

CRES, CST8, Cystatin 8

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using CST8 Polyclonal antibody (18183-1-AP)

    WB analysis of human testis using 18183-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 18183-1-AP (CST8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using CST8 Polyclonal antibody (18183-1-AP)

    WB analysis of DU 145 using 18183-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 18183-1-AP (CST8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CST8 Polyclonal antibody (18183-1-AP)

    WB analysis of HEK-293 using 18183-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18183-1-AP (CST8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman testis tissue, DU 145 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18183-1-AP targets CST8 in WB,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CST8 fusion protein Ag12896
Full Name cystatin 8 (cystatin-related epididymal specific)
Calculated Molecular Weight 142 aa, 16 kDa
Observed Molecular Weight 17-19 kDa, 9 kDa
GenBank Accession NumberBC069496
Gene Symbol CST8
Gene ID (NCBI) 10047
RRIDAB_10596471
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CST8 antibody 18183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols