CSTF1 Polyclonal antibody

CSTF1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 10064-2-AP

Synonyms

CF 1 50 kDa subunit, Cleavage stimulation factor, CstF 50, CSTF 50 kDa subunit, CSTF1, CstFp50



Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IP detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

10064-2-AP targets CSTF1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSTF1 fusion protein Ag0109
Full Name cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
Calculated Molecular Weight 48.3 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC001011
Gene Symbol CSTF1
Gene ID (NCBI) 1477
RRIDAB_2261145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cleavage stimulation factor (CstF) , a heterotrimeric protein with subunits of 77, 64, and 50 kD (CstF-77, -64, and -50), is a polyadenylation factor that helps to specify the site of processing. CstF recognizes the G+U-rich element, a sequence located downstream of the cleavage site. CstF-50 subunit interacts with the COOH-terminal domain of the RNAP II largest subunit (CTD). This subunit may be responsible for the interaction of CSTF with other factors to form a satable complex on the pre-mRNA, and multiple WD domains are required for protein interactions.

Protocols

Product Specific Protocols
WB protocol for CSTF1 antibody 10064-2-APDownload protocol
IP protocol for CSTF1 antibody 10064-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

The 3' ends of human pre-snRNAs are produced by RNA polymerase II CTD-dependent RNA processing.

Authors - Patricia Uguen
humanWB

Cell Rep

LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation

Authors - Nasha Zhang
humanWB

Oncogene

p53 inhibits mRNA 3' processing through its interaction with the CstF/BARD1 complex.

Authors - F I Nazeer

Andrology

Nicotine elevates sperm motility and induces Pfn1 promoter hypomethylation in mouse testis.

Authors - J Dai
rat,mouse,human

iScience

Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability

Authors - Ziqian Min