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CSTF3 Polyclonal antibody

CSTF3 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 24290-1-AP

Synonyms

CF 1 77 kDa subunit, CSTF 77, CSTF 77 kDa subunit, CSTF3

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using CSTF3 Polyclonal antibody (24290-1-AP)

    WB analysis of K-562 using 24290-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24290-1-AP (CSTF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using CSTF3 Polyclonal antibody (24290-1-AP)

    WB analysis of A431 using 24290-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 24290-1-AP (CSTF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using CSTF3 Polyclonal antibody (24290-1-AP)

    WB analysis of A431 using 24290-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 24290-1-AP (CSTF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using CSTF3 Polyclonal antibody (24290-1-AP)

    IP experiment of A431 using 24290-1-AP

    IP result of anti-CSTF3 (IP:24290-1-AP, 4ug; Detection:24290-1-AP 1:500) with A431 cells lysate 1200ug.

Tested Applications

Positive WB detected inK-562 cells, A431 cells
Positive IP detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24290-1-AP is CSTF3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSTF3 fusion protein Ag17930
Full Name cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa
Calculated Molecular Weight 717 aa, 83 kDa
Observed Molecular Weight 77-83 kDa
GenBank Accession NumberBC059948
Gene Symbol CSTF3
Gene ID (NCBI) 1479
RRIDAB_2879475
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CSTF3 antibody 24290-1-APDownload protocol
IP protocol for CSTF3 antibody 24290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF,IP

iScience

Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability

Authors - Ziqian Min
  • KD Validated
View Article