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CTBS Polyclonal antibody

CTBS Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12599-1-AP

Synonyms

chitobiase, di N acetyl, CTB, CTBS, Di N acetylchitobiase

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CTBS Polyclonal antibody (12599-1-AP)

    WB analysis of HeLa using 12599-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of SMMC-7721 cells using CTBS Polyclonal antibody (12599-1-AP)

    WB analysis of SMMC-7721 using 12599-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of MCF-7 cells using CTBS Polyclonal antibody (12599-1-AP)

    WB analysis of MCF-7 using 12599-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CTBS Polyclonal antibody (12599-1-AP)

    WB analysis of HepG2 using 12599-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using CTBS Polyclonal antibody (12599-1-AP)

    WB analysis of mouse colon using 12599-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CTBS Polyclonal antibody (12599-1-AP)

    WB analysis of HeLa using 12599-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CTBS Polyclonal antibody (12599-1-AP)

    WB analysis of HepG2 using 12599-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using CTBS Polyclonal antibody (12599-1-AP)

    WB analysis of mouse colon using 12599-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTBS Polyclonal antibody (12599-1-AP)

    IHC staining of human prostate cancer using 12599-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTBS Polyclonal antibody (12599-1-AP)

    IHC staining of human prostate cancer using 12599-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using CTBS Polyclonal antibody (12599-1-AP)

    IHC staining of human liver using 12599-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using CTBS Polyclonal antibody (12599-1-AP)

    IHC staining of human liver using 12599-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTBS Polyclonal antibody (12599-1-AP)

    IHC staining of human prostate cancer using 12599-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 12599-1-AP (CTBS Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CTBS Polyclonal antibody (12599-1-AP)

    IHC staining of human kidney using 12599-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CTBS Polyclonal antibody (12599-1-AP)

    IHC staining of human kidney using 12599-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTBS Polyclonal antibody (12599-1-AP)

    IHC staining of human prostate cancer using 12599-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12599-1-AP (CTBS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTBS Polyclonal antibody (12599-1-AP)

    IHC staining of human prostate cancer using 12599-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12599-1-AP (CTBS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CTBS Polyclonal antibody (12599-1-AP)

    IHC staining of human liver cancer using 12599-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12599-1-AP (CTBS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CTBS Polyclonal antibody (12599-1-AP)

    IHC staining of human liver cancer using 12599-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12599-1-AP (CTBS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CTBS Polyclonal antibody (12599-1-AP)

    IHC staining of human liver cancer using 12599-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CTBS Polyclonal antibody (12599-1-AP)

    IHC staining of human liver cancer using 12599-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CTBS Polyclonal antibody (12599-1-AP)

    IF Staining of HepG2 using 12599-1-AP

    Immunofluorescent analysis of HepG2 cells, using CTBS antibody 12599-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, SMMC-7721 cells, mouse colon tissue
Positive IHC detected inhuman prostate cancer tissue, human liver cancer tissue, human liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12599-1-AP targets CTBS in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTBS fusion protein Ag3228
Full Name chitobiase, di-N-acetyl-
Calculated Molecular Weight 12 kDa, 43 kDa
Observed Molecular Weight 44-50 kDa
GenBank Accession NumberBC024007
Gene Symbol CTBS
Gene ID (NCBI) 1486
RRIDAB_10638783
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTBS(Di-N-acetylchitobiase (chitobiase)) also named as CTB, is a lysosomal exoglycosidase that splits the GlcNAcβ-D-(l-4)GlcNAc chitobiose core of asparagine-linked glycoproteins. Chitobiase has been purified from human liver and kidney and rat liver. Both the human and rat enzymes are approximately 40-45 kDa and require a free reducing end GlcNAc for activity(PMID:1527079). This full length protein has a signal peptide and four glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for CTBS antibody 12599-1-APDownload protocol
IHC protocol for CTBS antibody 12599-1-APDownload protocol
IF protocol for CTBS antibody 12599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols