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  • Featured Product
  • KD/KO Validated

CTHRC1 Polyclonal antibody

CTHRC1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,IP,IF,ELISA

Conjugate

Unconjugated

Cat no : 16534-1-AP

Synonyms

CTHRC1, Protein NMTC1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CTHRC1 Polyclonal antibody (16534-1-AP)

    WB analysis using 16534-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16534-1-AP (CTHRC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using CTHRC1 Polyclonal antibody (16534-1-AP)

    WB analysis of A375 using 16534-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 16534-1-AP (CTHRC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat lung tissue using CTHRC1 Polyclonal antibody (16534-1-AP)

    WB analysis of rat lung using 16534-1-AP

    rat lung tissue were subjected to SDS PAGE followed by western blot with 16534-1-AP (CTHRC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CTHRC1 Polyclonal antibody (16534-1-AP)

    WB analysis of mouse lung using 16534-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16534-1-AP (CTHRC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CTHRC1 Polyclonal antibody (16534-1-AP)

    WB analysis of HEK-293 using 16534-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16534-1-AP (CTHRC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CTHRC1 Polyclonal antibody (16534-1-AP)

    WB analysis of Jurkat using 16534-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16534-1-AP (CTHRC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CTHRC1 Polyclonal antibody (16534-1-AP)

    WB analysis of mouse brain using 16534-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16534-1-AP (CTHRC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A375 cells using CTHRC1 Polyclonal antibody (16534-1-AP)

    IP experiment of A375 using 16534-1-AP

    IP result of anti-CTHRC1 (IP:16534-1-AP, 3ug; Detection:16534-1-AP 1:500) with A375 cells lysate 3600ug.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using CTHRC1 Polyclonal antibody (16534-1-AP)

    IF Staining of A375 using 16534-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A375 cells using 16534-1-AP (CTHRC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA375 cells, rat lung tissue, mouse lung tissue, HEK-293 cells, Jurkat cells, mouse brain tissue
Positive IP detected inA375 cells
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16534-1-AP targets CTHRC1 in WB,IP,IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTHRC1 fusion protein Ag9812
Full Name collagen triple helix repeat containing 1
Calculated Molecular Weight 243 aa, 26 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC014245
Gene Symbol CTHRC1
Gene ID (NCBI) 115908
RRIDAB_2087511
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Collagen triple helix repeat-containing protein 1 (CTHRC1), also known as protein NMTC1, is a glycosylated secreted protein. CTHRC1 was identified as a novel molecular in injured and diseased arteries where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration (PMID: 15618538). It has been reported that CTHRC1 is a positive regulator of osteoblastic bone formation (PMID: 18779865). Besides, studies reveal that CTHRC1 expression is implicated in various malignancies, including hepatocellular carcinoma, gastric carcinoma, colon cancer, and lung cancer (PMID: 23922981; 24504172; 24746208; 25139095).

Protocols

Product Specific Protocols
WB protocol for CTHRC1 antibody 16534-1-APDownload protocol
IF protocol for CTHRC1 antibody 16534-1-APDownload protocol
IP protocol for CTHRC1 antibody 16534-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Theranostics

Single-cell landscape reveals active cell subtypes and their interaction in the tumor microenvironment of gastric cancer.

Authors - Yumin Li
View Article
humanWB,IF

Respir Res

Pirfenidone attenuates lung fibrotic fibroblast responses to transforming growth factor-β1.

Authors - Jin Jin
View Article
humanWB

Int J Mol Sci

Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.

Authors - Federica Morani
View Article
ratWB,IHC

J Cell Sci

miR-9 inhibits Schwann cell migration by targeting CTHRC1 following sciatic nerve injury.

Authors - Songlin Zhou
  • KD Validated
View Article
human,mouseWB,IF

Inflammation

Collagen Triple Helix Repeat Containing 1 Deficiency Protects Against Airway Remodeling and Inflammation in Asthma Models In Vivo and In Vitro

Authors - Yong Feng
  • KD Validated
View Article
WB,IHC

Int J Biol Sci

NEDD4L-induced β-catenin ubiquitination suppresses the formation and progression of interstitial pulmonary fibrosis via inhibiting the CTHRC1/HIF-1α axis.

Authors - Lin Chen
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Valeria (Verified Customer) (11-11-2020)

Fixative: 4% paraformaldehydeAntigen retrieval step: NoPermeabilization: Methanol gradient (70% for 10 mins, 95% for 10 mins, 100% for 20 mins, 95% for 10 mins, 70% for 10 mins)Protein blocking: 10% NGS diluted in TBSTEndogenous biotin blocking: Avidin D solution for 15 mins, Biotin for 15 mins (Vector Laboratories, Inc)Secondary antibody: Life tech/ThermoFisher, A11006, Alexa 488, Goat anti-Rabbit isotype IgG, 1:100

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Human skeletal muscle sections (10um)
CTHRC1 Antibody Immunofluorescence validation (1:200 dilution) in Human skeletal muscle sections (10um) (Cat no:16534-1-AP)