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CWC15 Polyclonal antibody

CWC15 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 25293-1-AP

Synonyms

C11orf5, CWC15, Cwf15, HSPC148, ORF5, Protein CWC15 homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using CWC15 Polyclonal antibody (25293-1-AP)

    WB analysis of mouse liver using 25293-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using CWC15 Polyclonal antibody (25293-1-AP)

    WB analysis of mouse heart using 25293-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CWC15 Polyclonal antibody (25293-1-AP)

    WB analysis of Jurkat using 25293-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CWC15 Polyclonal antibody (25293-1-AP)

    WB analysis of HeLa using 25293-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using CWC15 Polyclonal antibody (25293-1-AP)

    WB analysis of PC-3 using 25293-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using CWC15 Polyclonal antibody (25293-1-AP)

    WB analysis of mouse testis using 25293-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CWC15 Polyclonal antibody (25293-1-AP)

    WB analysis of HepG2 using 25293-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SW 1990 cells using CWC15 Polyclonal antibody (25293-1-AP)

    WB analysis of SW 1990 using 25293-1-AP

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CWC15 Polyclonal antibody (25293-1-AP)

    IP experiment of HeLa using 25293-1-AP

    IP result of anti-CWC15 (IP:25293-1-AP, 3ug; Detection:25293-1-AP 1:600) with HeLa cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CWC15 Polyclonal antibody (25293-1-AP)

    IHC staining of human breast cancer using 25293-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25293-1-AP (CWC15 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using CWC15 Polyclonal antibody (25293-1-AP)

    IHC staining of human stomach using 25293-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25293-1-AP (CWC15 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using CWC15 Polyclonal antibody (25293-1-AP)

    IHC staining of human stomach using 25293-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25293-1-AP (CWC15 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CWC15 Polyclonal antibody (25293-1-AP)

    IHC staining of human breast cancer using 25293-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25293-1-AP (CWC15 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CWC15 Polyclonal antibody (25293-1-AP)

    IF Staining of HeLa using 25293-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 25293-1-AP (CWC15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells, HepG2 cells, Jurkat cells, mouse heart tissue, mouse testis tissue, PC-3 cells, SW 1990 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25293-1-AP is CWC15 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CWC15 fusion protein Ag18116
Full Name CWC15 spliceosome-associated protein homolog (S. cerevisiae)
Calculated Molecular Weight 229 aa, 27 kDa
Observed Molecular Weight 27-35 kDa
GenBank Accession NumberBC040946
Gene Symbol CWC15
Gene ID (NCBI) 51503
RRIDAB_2880012
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CWC15, also named as C11orf5 or Spliceosome-associated protein CWC15 homolog, is a 229 amino acid protein, which belongs to the CWC15 family. CWC15 as a Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.

Protocols

Product Specific Protocols
WB protocol for CWC15 antibody 25293-1-APDownload protocol
IHC protocol for CWC15 antibody 25293-1-APDownload protocol
IF protocol for CWC15 antibody 25293-1-APDownload protocol
IP protocol for CWC15 antibody 25293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nucleic Acids Res

CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex.

Authors - Febe van Maldegem
View Article