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CXXC1 Polyclonal antibody

CXXC1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC

Conjugate

Unconjugated

Cat no : 27963-1-AP

Synonyms

2410002I16Rik, 5830420C16Rik, CFP1, CGBP, CpG binding protein, CXXC finger 1 (PHD domain), CXXC1, hCGBP, HsT2645, PCCX1, PHF18, SPP1

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using CXXC1 Polyclonal antibody (27963-1-AP)

    WB analysis of K-562 using 27963-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 27963-1-AP (CXXC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CXXC1 Polyclonal antibody (27963-1-AP)

    WB analysis of Jurkat using 27963-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 27963-1-AP (CXXC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CXXC1 Polyclonal antibody (27963-1-AP)

    WB analysis of Jurkat using 27963-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 27963-1-AP (CXXC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CXXC1 Polyclonal antibody (27963-1-AP)

    IHC staining of human breast cancer using 27963-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27963-1-AP (CXXC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CXXC1 Polyclonal antibody (27963-1-AP)

    IHC staining of human breast cancer using 27963-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27963-1-AP (CXXC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inK-562 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27963-1-AP targets CXXC1 in WB, IHC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXXC1 fusion protein Ag27558
Full Name CXXC finger 1 (PHD domain)
Calculated Molecular Weight 76 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC015733
Gene Symbol CXXC1
Gene ID (NCBI) 30827
RRIDAB_2881023
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CXXC1 antibody 27963-1-APDownload protocol
IHC protocol for CXXC1 antibody 27963-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols