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KD/KO Validated

CXXC5 Polyclonal antibody

Name: CXXC5 Polyclonal antibody
Brand: Proteintech
SKU: 16513-1-AP

CXXC5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16513-1-AP

Synonyms

CXXC finger 5, CXXC5, CXXC-type zinc finger protein 5, HSPC195, Putative MAPK-activating protein PM08, Putative NF-kappa-B-activating protein 102, Retinoid-inducible nuclear factor, RINF, TCCCIA00297

Validation Data Gallery

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AllWBIPIHCIF

WB analysis of mouse brain using 16513-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 16513-1-AP (CXXC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 16513-1-PBS
MCF-7 cells were subjected to SDS PAGE followed by western blot with 16513-1-AP (CXXC5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of SH-SY5Y using 16513-1-AP
IP result of anti-CXXC5 (IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:8000) with SH-SY5Y cells lysate 1240 ug.
IP experiment of SH-SY5Y using 16513-1-AP
IP result of anti-CXXC5 (IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:300) with SH-SY5Y cells lysate 3400 ug.
IHC staining of human prostate cancer using 16513-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 16513-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16513-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16513-1-AP (CXXC5 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 16513-1-AP

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Tested Applications

Positive WB detected in	mouse brain tissue, MCF-7 cells
Positive IP detected in	SH-SY5Y cells
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 8 publications below
IHC	See 1 publications below
ChIP	See 4 publications below

Product Information

16513-1-AP targets CXXC5 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CXXC5 fusion protein Ag9733
Full Name	CXXC finger 5
Calculated Molecular Weight	322aa,33 kDa; 227aa,24 kDa
Observed Molecular Weight	35-40 kDa
GenBank Accession Number	BC017439
Gene Symbol	CXXC5
Gene ID (NCBI)	51523
RRID	AB_2878269
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXXC5 (CXXC-type zinc finger protein 5) is also named as HSPC195, RINF and TCCCIA00297.CXXC5 is a member of the CXXC-type zinc-finger protein family. CXXC5 is a short protein (322 amino acids in length) that does not have any catalytic domain, but is able to bind to DNA and act as a transcription factor and epigenetic factor through protein-protein interactions. In addition, CXXC5 is capable of regulating various signal transduction processes, including the TGF-β, Wnt and ATM-p53 pathways, thereby acting as a novel and crucial signaling coordinator. CXXC5 plays an important role in embryonic development and adult tissue homeostasis by regulating cell proliferation, differentiation and apoptosis (PMID: 30479059). CXXC5 has two isoforms of 33 kDa and 24 kDa (PMID: 25805812). CXXC5 is modified by phosphorylation after translation.

Protocols

Product Specific Protocols
WB protocol for CXXC5 antibody 16513-1-AP	Download protocol
IHC protocol for CXXC5 antibody 16513-1-AP	Download protocol
IF protocol for CXXC5 antibody 16513-1-AP	Download protocol
IP protocol for CXXC5 antibody 16513-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	ChIP,IHC,WB	Nat Commun A noncanonical AR addiction drives enzalutamide resistance in prostate cancer. Authors - Yundong He KD Validated View Article
mouse	WB	J Exp Med Epigenetic regulator CXXC5 recruits DNA demethylase Tet2 to regulate TLR7/9-elicited IFN response in pDCs. Authors - Shixin Ma KO Validated View Article
human	WB	Oncogene NUDT21 negatively regulates PSMB2 and CXXC5 by alternative polyadenylation and contributes to hepatocellular carcinoma suppression. Authors - Sheng Tan KD Validated View Article
human	WB,ChIP	Haematologica The epigenetic regulator RINF (CXXC5) maintains SMAD7 expression in human immature erythroid cells and sustains red blood cells expansion. Authors - Audrey Astori View Article
human	WB	Sci Rep KANK1 inhibits cell growth by inducing apoptosis though regulating CXXC5 in human malignant peripheral nerve sheath tumors. Authors - Zhibin Cui View Article
human,mouse	WB,ChIP	Front Cell Dev Biol Down-Regulation of CXXC5 De-Represses MYCL1 to Promote Hepatic Stellate Cell Activation. Authors - Xiaoyan Wu View Article

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CXXC5 Polyclonal antibody

CXXC5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis of mouse brain using 16513-1-AP

WB analysis of MCF-7 using 16513-1-PBS

IP experiment of SH-SY5Y using 16513-1-AP

IP experiment of SH-SY5Y using 16513-1-AP

IHC staining of human prostate cancer using 16513-1-AP

IHC staining of human prostate cancer using 16513-1-AP

IF Staining of HepG2 using 16513-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A noncanonical AR addiction drives enzalutamide resistance in prostate cancer.

Epigenetic regulator CXXC5 recruits DNA demethylase Tet2 to regulate TLR7/9-elicited IFN response in pDCs.

NUDT21 negatively regulates PSMB2 and CXXC5 by alternative polyadenylation and contributes to hepatocellular carcinoma suppression.

The epigenetic regulator RINF (CXXC5) maintains SMAD7 expression in human immature erythroid cells and sustains red blood cells expansion.

KANK1 inhibits cell growth by inducing apoptosis though regulating CXXC5 in human malignant peripheral nerve sheath tumors.

Down-Regulation of CXXC5 De-Represses MYCL1 to Promote Hepatic Stellate Cell Activation.