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  • Featured Product
  • KD/KO Validated

CYB5R3 Monoclonal antibody

CYB5R3 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

9C4D10

Cat no : 66022-1-Ig

Synonyms

B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CYB5R3 Monoclonal antibody (66022-1-Ig)

    WB analysis of HeLa using 66022-1-Ig

    WB results of CYB5R3 antibody (66022-1-Ig, 1:60000) with si-Control and si-CYB5R3 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using CYB5R3 Monoclonal antibody (66022-1-Ig)

    WB analysis of HeLa using 66022-1-Ig

    Western blot analysis of CYB5R3-specific in various tissues using Proteintech antibody 66022-1-Ig at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CYB5R3 Monoclonal antibody (66022-1-Ig)

    WB analysis of HeLa using 66022-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using CYB5R3 Monoclonal antibody (66022-1-Ig)

    WB analysis of human heart using 66022-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CYB5R3 Monoclonal antibody (66022-1-Ig)

    WB analysis of HepG2 using 66022-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using CYB5R3 Monoclonal antibody (66022-1-Ig)

    WB analysis of human heart using 66022-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using CYB5R3 Monoclonal antibody (66022-1-Ig)

    WB analysis of human plasma using 66022-1-Ig

    human blood were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using CYB5R3 Monoclonal antibody (66022-1-Ig)

    WB analysis of L02 using 66022-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYB5R3 Monoclonal antibody (66022-1-Ig)

    IHC staining of human liver cancer using 66022-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66022-1-Ig (CYB5R3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYB5R3 Monoclonal antibody (66022-1-Ig)

    IHC staining of human liver cancer using 66022-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66022-1-Ig (CYB5R3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using CYB5R3 Monoclonal antibody (66022-1-Ig)

    IHC staining of human liver using 66022-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using CYB5R3 Monoclonal antibody (66022-1-Ig)

    IHC staining of human liver using 66022-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CYB5R3 Monoclonal antibody (66022-1-Ig)

    IHC staining of human placenta using 66022-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CYB5R3 Monoclonal antibody (66022-1-Ig)

    IHC staining of human placenta using 66022-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CYB5R3 Monoclonal antibody (66022-1-Ig)

    IF Staining of HeLa using 66022-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYB5R3 antibody (66022-1-Ig, Clone: 9C4D10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CYB5R3 Monoclonal antibody (66022-1-Ig)

    IF Staining of HeLa using 66022-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYB5R3 antibody (66022-1-Ig, Clone: 9C4D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CYB5R3 Monoclonal antibody (66022-1-Ig)

    IF Staining of HepG2 using 66022-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, human heart tissue, human plasma tissue, HepG2 cells, L02 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66022-1-Ig targets CYB5R3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CYB5R3 fusion protein Ag18096
Full Name cytochrome b5 reductase 3
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC004821
Gene Symbol CYB5R3
Gene ID (NCBI) 1727
RRIDAB_11045662
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.

Protocols

Product Specific Protocols
WB protocol for CYB5R3 antibody 66022-1-IgDownload protocol
IHC protocol for CYB5R3 antibody 66022-1-IgDownload protocol
IF protocol for CYB5R3 antibody 66022-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols