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CYBRD1 Polyclonal antibody

CYBRD1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26735-1-AP

Synonyms

CYBRD1, cytochrome b reductase 1, DCYTB, Duodenal cytochrome b, Ferric chelate reductase 3, FRRS3

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using CYBRD1 Polyclonal antibody (26735-1-AP)

    WB analysis of COLO 320 using 26735-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 26735-1-AP (CYBRD1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CYBRD1 Polyclonal antibody (26735-1-AP)

    IHC staining of human breast cancer using 26735-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26735-1-AP (CYBRD1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CYBRD1 Polyclonal antibody (26735-1-AP)

    IHC staining of human breast cancer using 26735-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26735-1-AP (CYBRD1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CYBRD1 Polyclonal antibody (26735-1-AP)

    IHC staining of human lung cancer using 26735-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26735-1-AP (CYBRD1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CYBRD1 Polyclonal antibody (26735-1-AP)

    IHC staining of human lung cancer using 26735-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26735-1-AP (CYBRD1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26735-1-AP is CYBRD1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYBRD1 fusion protein Ag24968
Full Name cytochrome b reductase 1
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC065290
Gene Symbol CYBRD1
Gene ID (NCBI) 79901
RRIDAB_2880618
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CYBRD1 antibody 26735-1-APDownload protocol
IHC protocol for CYBRD1 antibody 26735-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Metallomics

Tolerable upper intake level of iron damages the intestine and alters the intestinal flora in weaned piglets.

Authors - Haoxuan Ding
View Article
mouseWB

Biol Trace Elem Res

Chronic Psychological Stress Disrupts Iron Metabolism and Enhances Hepatic Mitochondrial Function in Mice.

Authors - Shihui Guo
View Article
humanWB, IHC

PeerJ

Identification of potential genes in upper tract urothelial carcinoma using next-generation sequencing with bioinformatics and in vitro analyses.

Authors - Hsiang-Ying Lee
View Article