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  • Featured Product
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CYP20A1 Polyclonal antibody

CYP20A1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16702-1-AP

Synonyms

CYP M, CYP20A1, Cytochrome P450 20A1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CYP20A1 Polyclonal antibody (16702-1-AP)

    WB analysis of HEK-293 using 16702-1-AP

    WB result of CYP20A1 antibody (16702-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP20A1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using CYP20A1 Polyclonal antibody (16702-1-AP)

    WB analysis using 16702-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16702-1-AP (CYP20A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CYP20A1 Polyclonal antibody (16702-1-AP)

    WB analysis of MCF-7 using 16702-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16702-1-AP (CYP20A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CYP20A1 Polyclonal antibody (16702-1-AP)

    WB analysis of K-562 using 16702-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16702-1-AP (CYP20A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CYP20A1 Polyclonal antibody (16702-1-AP)

    WB analysis of mouse lung using 16702-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16702-1-AP (CYP20A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CYP20A1 Polyclonal antibody (16702-1-AP)

    WB analysis of HeLa using 16702-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16702-1-AP (CYP20A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using CYP20A1 Polyclonal antibody (16702-1-AP)

    IP experiment of MCF-7 using 16702-1-AP

    IP result of anti-CYP20A1 (IP:16702-1-AP, 4ug; Detection:16702-1-AP 1:300) with MCF-7 cells lysate 2400 ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP20A1 Polyclonal antibody (16702-1-AP)

    IHC staining of human liver cancer using 16702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16702-1-AP (CYP20A1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP20A1 Polyclonal antibody (16702-1-AP)

    IHC staining of human liver cancer using 16702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16702-1-AP (CYP20A1 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue, HEK-293 cells, K-562 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16702-1-AP targets CYP20A1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP20A1 fusion protein Ag10099
Full Name cytochrome P450, family 20, subfamily A, polypeptide 1
Calculated Molecular Weight 462 aa, 52 kDa
Observed Molecular Weight 45-52 kDa
GenBank Accession NumberBC020616
Gene Symbol CYP20A1
Gene ID (NCBI) 57404
RRIDAB_2261395
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CYP20A1 antibody 16702-1-APDownload protocol
IHC protocol for CYP20A1 antibody 16702-1-APDownload protocol
IP protocol for CYP20A1 antibody 16702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols