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CYP27A1 Polyclonal antibody

CYP27A1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 19195-1-AP

Synonyms

CP27, CTX, CYP27, CYP27A1, Cytochrome P 450C27/25, Cytochrome P450 27, Sterol 27 hydroxylase, Vitamin D(3) 25 hydroxylase

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using CYP27A1 Polyclonal antibody (19195-1-AP)

    WB analysis of L02 using 19195-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 19195-1-AP (CYP27A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CYP27A1 Polyclonal antibody (19195-1-AP)

    WB analysis of HepG2 using 19195-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19195-1-AP (CYP27A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CYP27A1 Polyclonal antibody (19195-1-AP)

    IHC staining of human kidney using 19195-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19195-1-AP (CYP27A1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CYP27A1 Polyclonal antibody (19195-1-AP)

    IHC staining of human kidney using 19195-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19195-1-AP (CYP27A1 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inL02 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

19195-1-AP targets CYP27A1 in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP27A1 fusion protein Ag7746
Full Name cytochrome P450, family 27, subfamily A, polypeptide 1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC051851
Gene Symbol CYP27A1
Gene ID (NCBI) 1593
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyzes oxidative cleavage of the sterol side chain in the bile acid biosynthetic pathway in the liver and 27-hydroxylation of cholesterol in most tissues(PMID:17088262). It belongs to the cytochrome P450 family. CYP27A1 catalyses an important sterol elimination pathway in the macrophage, and consequently may protect against atherosclerosis.

Publications

SpeciesApplicationTitle
mouseWB

Toxicol Sci

Hepatic, metabolic and toxicity evaluation of repeated oral administration of SnS2 nanoflowers in mice.

Authors - Disi Bai
View Article