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CYP39A1 Polyclonal antibody

CYP39A1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 16062-1-AP

Synonyms

CYP39A1, Cytochrome P450 39A1, hCYP39A1, Oxysterol 7 alpha hydroxylase

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using CYP39A1 Polyclonal antibody (16062-1-AP)

    WB analysis of L02 using 16062-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 16062-1-AP (CYP39A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using CYP39A1 Polyclonal antibody (16062-1-AP)

    WB analysis of SMMC-7721 using 16062-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16062-1-AP (CYP39A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP39A1 Polyclonal antibody (16062-1-AP)

    IHC staining of human liver using 16062-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16062-1-AP (CYP39A1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP39A1 Polyclonal antibody (16062-1-AP)

    IHC staining of human liver using 16062-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16062-1-AP (CYP39A1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP39A1 Polyclonal antibody (16062-1-AP)

    IHC staining of human liver cancer using 16062-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16062-1-AP (CYP39A1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP39A1 Polyclonal antibody (16062-1-AP)

    IHC staining of human liver cancer using 16062-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16062-1-AP (CYP39A1 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inL02 cells, SMMC-7721 cells
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16062-1-AP targets CYP39A1 in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP39A1 fusion protein Ag8896
Full Name cytochrome P450, family 39, subfamily A, polypeptide 1
Calculated Molecular Weight 469 aa, 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC010358
Gene Symbol CYP39A1
Gene ID (NCBI) 51302
RRIDAB_2878211
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CYP39A1 antibody 16062-1-APDownload protocol
IHC protocol for CYP39A1 antibody 16062-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols