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CYP3A5 Polyclonal antibody

CYP3A5 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13737-1-AP

Synonyms

CP35, CYP3A5, CYPIIIA5, Cytochrome P450 3A5, Cytochrome P450 HLp2, Cytochrome P450 PCN3, P450PCN3, PCN3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using CYP3A5 Polyclonal antibody (13737-1-AP)

    WB analysis of mouse liver using 13737-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 13737-1-AP (CYP3A5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using CYP3A5 Polyclonal antibody (13737-1-AP)

    WB analysis of mouse liver using 13737-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 13737-1-AP (CYP3A5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using CYP3A5 Polyclonal antibody (13737-1-AP)

    WB analysis of human liver using 13737-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13737-1-AP (CYP3A5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CYP3A5 Polyclonal antibody (13737-1-AP)

    WB analysis of HEK-293 using 13737-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13737-1-AP (CYP3A5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP3A5 Polyclonal antibody (13737-1-AP)

    IHC staining of human liver cancer using 13737-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13737-1-AP (CYP3A5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP3A5 Polyclonal antibody (13737-1-AP)

    IHC staining of human liver cancer using 13737-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13737-1-AP (CYP3A5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue, human liver tissue, HEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

13737-1-AP targets CYP3A5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP3A5 fusion protein Ag4674
Full Name cytochrome P450, family 3, subfamily A, polypeptide 5
Calculated Molecular Weight 502 aa, 57 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC033862
Gene Symbol CYP3A5
Gene ID (NCBI) 1577
RRIDAB_2090516
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CYP3A5 antibody 13737-1-APDownload protocol
IHC protocol for CYP3A5 antibody 13737-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Oncol

Novel Prognostic Signatures of Hepatocellular Carcinoma Based on Metabolic Pathway Phenotypes.

Authors - Tingbo Ye
View Article

Oncotarget

Alpha-ketoglutarate suppresses the NF-κB-mediated inflammatory pathway and enhances the PXR-regulated detoxification pathway.

Authors - Liuqin He
View Article