CYP4A11 Polyclonal antibody

CYP4A11 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11688-1-AP

Synonyms

20 HETE synthase, CP4Y, CYP4A11, CYP4A2, CYP4AII, CYPIVA11, Cytochrome P 450HK omega, Cytochrome P450 4A11, Cytochrome P450HL omega, Fatty acid omega hydroxylase, Lauric acid omega hydroxylase



Tested Applications

Positive WB detected inHEK-293 cells, rat kidney tissue, mouse kidney tissue
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:300-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

11688-1-AP targets CYP4A11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP4A11 fusion protein Ag2300
Full Name cytochrome P450, family 4, subfamily A, polypeptide 11
Calculated Molecular Weight 519 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC022851
Gene Symbol CYP4A11
Gene ID (NCBI) 1579
RRIDAB_10804774
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CYP4A11 antibody 11688-1-APDownload protocol
IHC protocol for CYP4A11 antibody 11688-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Free Radic Biol Med

PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase.

Authors - Xue Chen
ratWB

Food Funct

Correction: Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.

Authors - Deshi Dong
ratWB

Food Funct

Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.

Authors - Deshi Dong
humanWB

Forensic Sci Int

Identification of the miRNA-3185/CYP4A11 axis in cardiac tissue as a biomarker for mechanical asphyxia.

Authors - Liujun Han
mouse,ratWB

Chem Biol Interact

Activation of 20-HETE/PPARs involved in reno-therapeutic effect of naringenin on diabetic nephropathy.

Authors - Shumei Ding