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Calsequestrin 1 Polyclonal antibody

Calsequestrin 1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26665-1-AP

Synonyms

Calmitin, Calsequestrin 1, CASQ, CASQ1, PDIB1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse heart tissue using Calsequestrin 1 Polyclonal antibody (26665-1-AP)

    WB analysis of mouse heart using 26665-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 26665-1-AP (Calsequestrin antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Calsequestrin 1 Antibody IHC human skeletal muscle tissue 26665-1-AP

    IHC staining of human skeletal muscle tissue using 26665-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26665-1-AP( Calsequestrin antibody) at dilution of 1:200 (under 10x lens).

  • Calsequestrin 1 Antibody IHC human skeletal muscle tissue 26665-1-AP

    IHC staining of human skeletal muscle tissue using 26665-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26665-1-AP( Calsequestrin antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26665-1-AP is Calsequestrin 1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calsequestrin 1 fusion protein Ag24684
Full Name calsequestrin 1 (fast-twitch, skeletal muscle)
Calculated Molecular Weight 390 aa, 45 kDa
Observed Molecular Weight 45 kDa, 63 kDa
GenBank Accession NumberBC022289
Gene Symbol CASQ1
Gene ID (NCBI) 844
RRIDAB_2880594
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Calsequestrin 1 antibody 26665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Mol Biosci

Altered Glycosylation in the Aging Heart.

Authors - Patricia Franzka
View Article
humanIHC

J Neuropathol Exp Neurol

Morphological Alterations of the Sarcotubular System in Permanent Myopathy of Hereditary Hypokalemic Periodic Paralysis with a Mutation in the CACNA1S Gene.

Authors - Takamura Nagasaka
View Article
mouseWB

J Muscle Res Cell Motil

Differential regulation of Actn2 and Actn3 expression during unfolded protein response in C2C12 myotubes.

Authors - Nagakatsu Harada
View Article
mouseWB

JCI Insight

Critical role of Znhit1 for postnatal heart function and vacuolar cardiomyopathy.

Authors - Yingchao Shi
View Article