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Collagen Type V Polyclonal antibody

Collagen Type V Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 28382-1-AP

Synonyms

COL5A1, Collagen alpha 1(V) chain, Collagen Type V, collagen, type V, alpha 1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon tissue using Collagen Type V Polyclonal antibody (28382-1-AP)

    IHC staining of human colon using 28382-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Collagen Type V Polyclonal antibody (28382-1-AP)

    IHC staining of human colon using 28382-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Collagen Type V Polyclonal antibody (28382-1-AP)

    IHC staining of human lung cancer using 28382-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Collagen Type V Polyclonal antibody (28382-1-AP)

    IHC staining of human lung cancer using 28382-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Collagen Type V Polyclonal antibody (28382-1-AP)

    IHC staining of human placenta using 28382-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Collagen Type V Polyclonal antibody (28382-1-AP)

    IHC staining of human placenta using 28382-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman colon tissue, human lung cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28382-1-AP targets Collagen Type V in IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Purified native protein corresponding to human collagen type V
Full Name collagen, type V, alpha 1
Calculated Molecular Weight 184 kDa
GenBank Accession NumberNM_000093
Gene Symbol COL5A1
Gene ID (NCBI) 1289
RRIDAB_2881129
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for Collagen Type V antibody 28382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Liliana (Verified Customer) (02-10-2024)

heat induced pretreatment EDTA, ph9

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:4000
  • Cell Tissue Type: skin, keloid scar
FH

Liliana (Verified Customer) (02-10-2024)

heat induced pre-treatment, EDTA, ph9 worked very well on keloid scar, normal skin, scleroderma, lichen slerosus

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: normal skin