Cystatin B Monoclonal antibody

Cystatin B Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2G9B8

Cat no : 66812-1-Ig

Synonyms

CPI B, CST6, CSTB, Cystatin B, cystatin B (stefin B), EPM1, PME, Stefin B, STFB



Tested Applications

Positive WB detected inU-937 cells, human saliva tissue, THP-1 cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66812-1-Ig targets Cystatin B in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cystatin B fusion protein Ag21291
Full Name cystatin B (stefin B)
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 11-14 kDa
GenBank Accession NumberBC003370
Gene Symbol CSTB
Gene ID (NCBI) 1476
RRIDAB_2882155
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cystatin B (CSTB), a member of the cystatin superfamily protein, is a stefin that functions as an intracellular thiol protease inhibitor and has been thought to play a role in protecting against the proteases leaking from lysosomes. CSTB plays various functions in a variety of diseases, including epithelial ovarian cancer, colon cancer, and myoclonus epilepsy. Evidence indicates that mutations in CSTB are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.

Protocols

Product Specific Protocols
WB protocol for Cystatin B antibody 66812-1-IgDownload protocol
IF protocol for Cystatin B antibody 66812-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols