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Cytokeratin 8 Monoclonal antibody

Cytokeratin 8 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E10A10

Cat no : 60274-1-Ig

Synonyms

CARD2, Cell and organelle markers, CK 8, CK8, CYK8, Cytokeratin 8, Cytoskeleton Marker, K2C8, K8, keratin 8, KO, KRT8, Type II keratin Kb8

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using Cytokeratin 8 Monoclonal antibody (60274-1-Ig)

    WB analysis of A549 using 60274-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 60274-1-Ig (Cytokeratin 8 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60274-1-Ig targets Cytokeratin 8 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name keratin 8
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberNM_002273
Gene Symbol KRT8
Gene ID (NCBI) 3856
RRIDAB_2881394
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 8 antibody 60274-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols