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CoraLite®594-conjugated DACH1 Polyclonal antibody

DACH1 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10914

Synonyms

DACH, DACH1, Dachshund homolog 1, FLJ10138

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite®594-conjugated DACH1 Polyclonal antibody (CL594-10914)

    IF Staining of HEK-293 using CL594-10914

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite®594 DACH1 antibody (CL594-10914) at dilution of 1:200.

Tested Applications

Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10914 targets DACH1 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DACH1 fusion protein Ag1354
Full Name dachshund homolog 1 (Drosophila)
Calculated Molecular Weight 79 kDa
GenBank Accession NumberBC021219
Gene Symbol DACH1
Gene ID (NCBI) 1602
RRIDAB_2919754
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human DACH1.

Protocols

Product Specific Protocols
IF protocol for CL594 DACH1 antibody CL594-10914Download protocol
Standard Protocols
Click here to view our Standard Protocols