DAZL Polyclonal antibody

DAZL Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12633-1-AP

Synonyms

DAZ homolog, DAZ like autosomal, DAZH, DAZL, DAZL1, DAZLA, deleted in azoospermia like, Deleted in azoospermia like 1, SPGY like autosomal, SPGYLA



Tested Applications

Positive WB detected inrat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12633-1-AP targets DAZL in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAZL fusion protein Ag3326
Full Name deleted in azoospermia-like
Calculated Molecular Weight 295 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC027595
Gene Symbol DAZL
Gene ID (NCBI) 1618
RRIDAB_2277011
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DAZL antibody 12633-1-APDownload protocol
IHC protocol for DAZL antibody 12633-1-APDownload protocol
IF protocol for DAZL antibody 12633-1-APDownload protocol
IP protocol for DAZL antibody 12633-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Environ Pollut

Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling

Authors - Yifan Hong
ratIF

Stem Cell Res Ther

Concentrated exosomes from menstrual blood-derived stromal cells improves ovarian activity in a rat model of premature ovarian insufficiency.

Authors - Siwen Zhang
human,mouseWB

Proteomics

Systematic in-depth proteomic analysis of mitochondria-associated endoplasmic reticulum membranes (MAM) in mouse and human testes.

Authors - Xiaoli Wang
mouseWB

Front Endocrinol (Lausanne)

Restoring Impaired Fertility Through Diet: Observations of Switching From High-Fat Diet During Puberty to Normal Diet in Adulthood Among Obese Male Mice.

Authors - Xiangyu Qi