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DBI Polyclonal antibody

DBI Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14490-1-AP

Synonyms

ACBD1, ACBP, Acyl CoA binding protein, CCK RP, DBI, Diazepam binding inhibitor, Endozepine, EP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DBI Polyclonal antibody (14490-1-AP)

    WB analysis using 14490-1-AP

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 14490-1-AP (DBI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using DBI Polyclonal antibody (14490-1-AP)

    WB analysis of human liver using 14490-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 14490-1-AP (DBI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inU-87 MG cells, human liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14490-1-AP targets DBI in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DBI fusion protein Ag5891
Full Name diazepam binding inhibitor (GABA receptor modulator, acyl-Coenzyme A binding protein)
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC062996
Gene Symbol DBI
Gene ID (NCBI) 1622
RRIDAB_2211037
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DBI antibody 14490-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Sci Rep

Thioesterase induction by 2,3,7,8-tetrachlorodibenzo-p-dioxin results in a futile cycle that inhibits hepatic β-oxidation.

Authors - Giovan N Cholico
View Article
mouseWB

Eur Neuropsychopharmacol

Deficiency of prolyl oligopeptidase in mice disturbs synaptic plasticity and reduces anxiety-like behaviour, body weight, and brain volume.

Authors - Corinna Höfling
View Article