DBT Polyclonal antibody

DBT Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 12451-1-AP

Synonyms

DBT, E2, E2B



Tested Applications

Positive WB detected inHepG2 cells, mouse brain tissue, rat brain tissue, NCI-H1299 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1500-1:6000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12451-1-AP targets DBT in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DBT fusion protein Ag3101
Full Name dihydrolipoamide branched chain transacylase E2
Calculated Molecular Weight 482 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC016675
Gene Symbol DBT
Gene ID (NCBI) 1629
RRIDAB_2089636
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DBT antibody 12451-1-APDownload protocol
IF protocol for DBT antibody 12451-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Metab

Branched-chain amino acid catabolism in muscle affects systemic BCAA levels but not insulin resistance

Authors - Megan C Blair
mouseWB,IP

J Nutr

Pyruvate Kinase M2 Supports Muscle Progenitor Cell Proliferation but Is Dispensable for Skeletal Muscle Regeneration after Injury.

Authors - Jamie E Blum
human,mouseWB

Heliyon

Hyperactivation of mTOR and AKT in a cardiac hypertrophy animal model of Friedreich ataxia

Authors - Wing-Hang Tong
mouseWB

Cell Metab

Extra-cardiac BCAA catabolism lowers blood pressure and protects from heart failure

Authors - Danielle Murashige
humanWB

Front Immunol

Overexpression of DBT suppresses the aggressiveness of renal clear cell carcinoma and correlates with immune infiltration

Authors - Chiyu Zhang
mouseWB

J Thromb Haemost

Branched-chain amino acids promote thrombocytopoiesis by activating mTOR signaling

Authors - Haojie Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Maria (Verified Customer) (08-15-2021)

DBT in human primary fibroblasts. 10 ug of total protein. 4-15% TGX gel. Blocking 3% BSA in PBST (0.1% Tween). 1:1000 primary ab O/N incubation at 4ºC. 1:5000 secondary ab HRP conj. 1h RT in BSA 3% in PBST.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human primary fibroblasts
DBT Antibody Western Blot validation (1:1000 dilution) in Human primary fibroblasts (Cat no:12451-1-AP)