DCDC2 Polyclonal antibody

DCDC2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26978-1-AP

Synonyms

DCDC2, DCDC2A, KIAA1154, Protein RU2S, RU2, RU2S



Tested Applications

Positive WB detected inmouse placenta tissue, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26978-1-AP targets DCDC2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCDC2 fusion protein Ag25577
Full Name doublecortin domain containing 2
Calculated Molecular Weight 476 aa, 53 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC050704
Gene Symbol DCDC2
Gene ID (NCBI) 51473
RRIDAB_2880709
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DCDC2 antibody 26978-1-APDownload protocol
IHC protocol for DCDC2 antibody 26978-1-APDownload protocol
IF protocol for DCDC2 antibody 26978-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Physiol Genomics

Polycystin-2-dependent transcriptome reveals early response of autosomal dominant polycystic kidney disease

Authors - Hyun Jun Jung