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  • KD/KO Validated

DDX1 Polyclonal antibody

DDX1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11357-1-AP

Synonyms

DBP RB, DDX1, DEAD box protein 1, UKVH5d



Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, HeLa cells, MCF-7 cells, NCI-H1299 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue, PC-3 cells, mouse kidney tissue, rat kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11357-1-AP targets DDX1 in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX1 fusion protein Ag1887
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
Calculated Molecular Weight 740 aa, 82 kDa
Observed Molecular Weight70-82 kDa
GenBank Accession NumberBC012132
Gene Symbol DDX1
Gene ID (NCBI) 1653
RRIDAB_2092222
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX1 is a DEAD box protein, which is putative RNA helicases with a characteristic asp-glu-ala-asp (DEAD) box motif. DEAD box proteins involve in translation initiation, splicing, and ribosome and spliceosome assembly by altering RNA secondary structure. As a RNA helicase, DDX1 has a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome.

Protocols

Product Specific Protocols
WB protocol for DDX1 antibody 11357-1-APDownload protocol
IHC protocol for DDX1 antibody 11357-1-APDownload protocol
IP protocol for DDX1 antibody 11357-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell

Modulation of RNA Condensation by the DEAD-Box Protein eIF4A.

Authors - Devin Tauber
mouseWB,CoIP,IP

Mol Cell

RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination.

Authors - Claudia Ribeiro de Almeida
  • KD Validated
humanWB

Cell Host Microbe

RNase L Activates the NLRP3 Inflammasome during Viral Infections.

Authors - Arindam Chakrabarti
  • KD Validated
mouseWB

Nat Commun

BEX1 is an RNA-dependent mediator of cardiomyopathy.

Authors - Federica Accornero
ratWB,IF

Nucleic Acids Res

PMID: 30295850

Authors - Zonghong Li
  • KD Validated
mouseIF

Cell Syst

BraInMap Elucidates the Macromolecular Connectivity Landscape of Mammalian Brain.

Authors - Reza Pourhaghighi