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DDX4/VASA Polyclonal antibody

DDX4,VASA Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

ELISA

Conjugate

Unconjugated

Cat no : 12888-1-AP

Synonyms

DDX4, DEAD box protein 4, VASA, Vasa homolog

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using DDX4/VASA Polyclonal antibody (12888-1-AP)

    IHC staining of mouse testis using 12888-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12888-1-AP (DDX4/VASA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DDX4/VASA Polyclonal antibody (12888-1-AP)

    IHC staining of mouse testis using 12888-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12888-1-AP (DDX4/VASA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12888-1-AP targets DDX4,VASA in ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX4,VASA fusion protein Ag3919
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Calculated Molecular Weight690aa,76 kDa; 724aa,79 kDa
GenBank Accession NumberBC047455
Gene Symbol DDX4
Gene ID (NCBI) 54514
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].