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DECR2 Polyclonal antibody

DECR2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 25855-1-AP

Synonyms

2,4 dienoyl CoA reductase 2, DECR2, PDCR, SDR17C1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using DECR2 Polyclonal antibody (25855-1-AP)

    WB analysis of mouse lung using 25855-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 25855-1-AP (DECR2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse lung tissue using DECR2 Polyclonal antibody (25855-1-AP)

    WB analysis of mouse lung using 25855-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 25855-1-AP (DECR2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using DECR2 Polyclonal antibody (25855-1-AP)

    IHC staining of mouse lung using 25855-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25855-1-AP (DECR2 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using DECR2 Polyclonal antibody (25855-1-AP)

    IHC staining of mouse lung using 25855-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25855-1-AP (DECR2 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25855-1-AP is DECR2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DECR2 fusion protein Ag23052
Full Name 2,4-dienoyl CoA reductase 2, peroxisomal
Calculated Molecular Weight 292 aa, 31 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC011968
Gene Symbol DECR2
Gene ID (NCBI) 26063
RRIDAB_2880270
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DECR2 antibody 25855-1-APDownload protocol
IHC protocol for DECR2 antibody 25855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Frauke (Verified Customer) (09-27-2021)

Clear signal, low background works on yeast postnuclear supernatant

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: yeast post nuclear supernatant