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DEFA1 Polyclonal antibody

DEFA1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, CoIP

Conjugate

Unconjugated

Cat no : 18057-1-AP

Synonyms

DEF1, DEFA1, DEFA1B, DEFA2, defensin, alpha 1, HNP 1, HNP 2, HP 1, HP 2, HP1, HP2, MRS, Neutrophil defensin 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using DEFA1 Polyclonal antibody (18057-1-AP)

    WB analysis of HepG2 using 18057-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18057-1-AP (DEFA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using DEFA1 Polyclonal antibody (18057-1-AP)

    IHC staining of human small intestine using 18057-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18057-1-AP (DEFA1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using DEFA1 Polyclonal antibody (18057-1-AP)

    IHC staining of human small intestine using 18057-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18057-1-AP (DEFA1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DEFA1 Polyclonal antibody (18057-1-AP)

    IHC staining of human liver cancer using 18057-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18057-1-AP (DEFA1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DEFA1 Polyclonal antibody (18057-1-AP)

    IHC staining of human liver cancer using 18057-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18057-1-AP (DEFA1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DEFA1 Polyclonal antibody (18057-1-AP)

    IF Staining of HepG2 using 18057-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 18057-1-AP (DEFA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman small intestine tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18057-1-AP targets DEFA1 in WB, IHC, IF, CoIP applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DEFA1 fusion protein Ag12592
Full Name defensin, alpha 1
Calculated Molecular Weight 94 aa, 10 kDa
Observed Molecular Weight 6 kDa
GenBank Accession NumberBC093791
Gene Symbol DEFA1
Gene ID (NCBI) 1667
RRIDAB_2292842
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DEFA1 antibody 18057-1-APDownload protocol
IHC protocol for DEFA1 antibody 18057-1-APDownload protocol
IF protocol for DEFA1 antibody 18057-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Life Sci

DEFA1B inhibits ZIKV replication and retards cell cycle progression through interaction with ORC1.

Authors - Shuang Li
View Article
humanWB

Hum Cell

Histone deacetylase 6-mediated downregulation of TMEM100 expedites the development and progression of non-small cell lung cancer.

Authors - Yanyun Wang
View Article
humanIHC

Syst Biol Reprod Med

Identification of differentially expressed proteins associated with recurrence in ovarian endometriotic cysts.

Authors - Genhai Zhu
View Article