Desmin Polyclonal antibody

Desmin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 16520-1-AP

Synonyms

CMD1I, CSM1, CSM2, DES, desmin



Tested Applications

Positive WB detected inC2C12 cells, human heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, human appendix tissue, human skeletal muscle tissue, human placenta tissue, human colon tissue, human hysteromyoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC2C12 cell, C2C12 cells, mouse heart tissue
Positive FC detected inC2C12 cell
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16520-1-AP targets Desmin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Desmin fusion protein Ag9742
Full Name desmin
Calculated molecular weight 470 aa, 54 kDa
Observed molecular weight 52-53 kDa
GenBank accession numberBC032116
Gene symbol DES
Gene ID (NCBI) 1674
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Protocols

Product Specific Protocols
WB protocol for Desmin antibody 16520-1-APDownload protocol
IHC protocol for Desmin antibody 16520-1-APDownload protocol
IF protocol for Desmin antibody 16520-1-APDownload protocol
IP protocol for Desmin antibody 16520-1-APDownload protocol
FC protocol for Desmin antibody 16520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

J Cell Mol Med

miR-193a/b-3p relieves hepatic fibrosis and restrains proliferation and activation of hepatic stellate cells.

Authors - Baoling Ju
mouseWB

Cell Death Dis

Yes-associated protein regulates podocyte cell cycle re-entry and dedifferentiation in adriamycin-induced nephropathy.

Authors - Kewei Xie

Scand J Immunol

Interleukin-17A participates in podocyte injury by inducing IL-1β secretion through ROS-NLRP3 inflammasome-caspase-1 pathway.

Authors - J Yan
rat,humanIHC

Clin Exp Nephrol

Neointimal hyperplasia in the inferior vena cava of adenine-induced chronic kidney disease rats with aortocaval fistulas.

Authors - Jing Du
mouseWB,IP

Genes Cells

Multimeric conformation of type III intermediate filaments but not the filamentous conformation exhibits high affinity to lipid bilayers.

Authors - Beomju Hwang
ratWB

Life Sci

Effects of asiaticoside on levels of podocyte cytoskeletal proteins and renal slit diaphragm proteins in adriamycin-induced rat nephropathy.

Authors - Wang Zhu Z

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sarah (Verified Customer) (11-16-2019)

This antibody worked well for both WB (1:4000 for 1 hour) and IP (1.6ug Ab/ 500ug Protein)

  • Applications: Western Blot,Immunoprecipitation,
  • Primary Antibody Dilution: WB- 1:4000, IP- 1.6ug
  • Cell Tissue Type: C2C12 Cells
Desmin Antibody Western Blot,Immunoprecipitation, validation (WB- 1:4000, IP- 1.6ug dilution) in C2C12 Cells (Cat no:16520-1-AP)