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Desmin Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 16520-1-AP

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Con: 60 μg/150 μl

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Tested Applications
Applications:
ELISA, WB, IP, IHC, IF, FC
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Images See all

16520-1-AP;mouse heart tissue

mouse heart tissue were subjected to SDS PAGE followed by western blot with 16520-1-AP(DES antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours

16520-1-AP;human heart tissue

Immunohistochemistry of paraffin-embedded human heart tissue slide using 16520-1-AP( Desmin Antibody) at dilution of 1:200 (under 10x lens).

16520-1-AP;human heart tissue

Immunohistochemistry of paraffin-embedded human heart tissue slide using 16520-1-AP( Desmin Antibody) at dilution of 1:200 (under 40x lens).

16520-1-AP;mouse heart tissue

IP Result of anti-Desmin (IP:16520-1-AP, 4ug; Detection:16520-1-AP 1:3000) with mouse heart tissue lysate 8000ug.

16520-1-AP;C2C12 cell

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed C2C12 cell using 16520-1-AP(Desmin antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

16520-1-AP;C2C12 cell

1X10^6 C2C12 cell were stained with 0.2ug Desmin antibody (16520-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse heart tissue, human heart tissue, mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IP detected in:
mouse heart tissue
Positive IHC detected in:
human heart tissue, human appendix tissue, human colon tissue, human heart tissue, human hysteromyoma tissue, human placenta tissue, human skeletal muscle tissue, human testis tissue, rat heart tissue
Positive IF detected in:
C2C12 cell
Positive FC detected in:
C2C12 cell
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Desmin fusion protein ag9742
Full name:
desmin
Calculated molecular weight:
470aa,54 kDa
Observed molecular weight:
52-53 kDa
GenBank accession number:
BC032116
Gene ID (NCBI):
1674
Gene symbol
DES
Synonyms
CMD1I, CSM1, CSM2, DES, desmin, FLJ12025, FLJ39719, FLJ41013, FLJ41793
Background

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.


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