Desmin Polyclonal antibody
Desmin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 16520-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | C2C12 cells, human heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human heart tissue, human appendix tissue, human skeletal muscle tissue, human placenta tissue, human colon tissue, human hysteromyoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | C2C12 cell, C2C12 cells, mouse heart tissue |
| Positive FC detected in | C2C12 cell |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 11 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
Product Information
16520-1-AP targets Desmin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Desmin fusion protein Ag9742 |
| Full Name | desmin |
| Calculated molecular weight | 470 aa, 54 kDa |
| Observed molecular weight | 52-53 kDa |
| GenBank accession number | BC032116 |
| Gene symbol | DES |
| Gene ID (NCBI) | 1674 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Desmin antibody 16520-1-AP | Download protocol |
| IHC protocol for Desmin antibody 16520-1-AP | Download protocol |
| IF protocol for Desmin antibody 16520-1-AP | Download protocol |
| IP protocol for Desmin antibody 16520-1-AP | Download protocol |
| FC protocol for Desmin antibody 16520-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Mol Med miR-193a/b-3p relieves hepatic fibrosis and restrains proliferation and activation of hepatic stellate cells. | ||
Cell Death Dis Yes-associated protein regulates podocyte cell cycle re-entry and dedifferentiation in adriamycin-induced nephropathy. | ||
Scand J Immunol Interleukin-17A participates in podocyte injury by inducing IL-1β secretion through ROS-NLRP3 inflammasome-caspase-1 pathway. | ||
Clin Exp Nephrol Neointimal hyperplasia in the inferior vena cava of adenine-induced chronic kidney disease rats with aortocaval fistulas. | ||
Genes Cells Multimeric conformation of type III intermediate filaments but not the filamentous conformation exhibits high affinity to lipid bilayers. | ||
Life Sci Effects of asiaticoside on levels of podocyte cytoskeletal proteins and renal slit diaphragm proteins in adriamycin-induced rat nephropathy. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sarah (Verified Customer) (11-16-2019) | This antibody worked well for both WB (1:4000 for 1 hour) and IP (1.6ug Ab/ 500ug Protein)
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