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Desmin Antibody 8 Publications

Rabbit Polyclonal| Catalog number: 16520-1-AP

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Con: 60 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

Desmin Antibody IHC human colon tissue 16520-1-AP

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16520-1-AP (Desmin antibody) at dilution of 1:12800 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Desmin Antibody WB mouse heart tissue 16520-1-AP

mouse heart tissue were subjected to SDS PAGE followed by western blot with 16520-1-AP(DES antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours

Desmin Antibody IHC human heart tissue 16520-1-AP

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16520-1-AP( Desmin Antibody) at dilution of 1:200 (under 10x lens).

Desmin Antibody IHC human heart tissue 16520-1-AP

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16520-1-AP( Desmin Antibody) at dilution of 1:200 (under 40x lens).

Desmin Antibody IP mouse heart tissue 16520-1-AP

IP Result of anti-Desmin (IP:16520-1-AP, 4ug; Detection:16520-1-AP 1:3000) with mouse heart tissue lysate 8000ug.

Desmin Antibody IF C2C12 cell 16520-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed C2C12 cell using 16520-1-AP(Desmin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Desmin Antibody FC C2C12 cell 16520-1-AP

1X10^6 C2C12 cell were stained with 0.2ug Desmin antibody (16520-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse heart tissue, human heart tissue, rat heart tissue
Positive IP detected in:
mouse heart tissue
Positive IHC detected in:
human heart tissue, human colon tissue, human appendix tissue, human hysteromyoma tissue, human skeletal muscle tissue, human placenta tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
C2C12 cell
Positive FC detected in:
C2C12 cell
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Desmin fusion protein Ag9742
Full name:
desmin
Calculated molecular weight:
470aa,54 kDa
Observed molecular weight:
52-53 kDa
GenBank accession number:
BC032116
Gene ID (NCBI):
1674
Gene symbol
DES
Synonyms
CMD1I, CSM1, CSM2, DES, desmin

Background

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.


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