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dGK Polyclonal antibody

dGK Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 16314-1-AP

Synonyms

Deoxyguanosine kinase, dGK, DGUOK

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using dGK Polyclonal antibody (16314-1-AP)

    WB analysis of mouse kidney using 16314-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16314-1-AP (dGK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using dGK Polyclonal antibody (16314-1-AP)

    WB analysis of mouse kidney using 16314-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16314-1-AP (dGK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using dGK Polyclonal antibody (16314-1-AP)

    IHC staining of human hepatocirrhosis using 16314-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 16314-1-AP (dGK antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using dGK Polyclonal antibody (16314-1-AP)

    IHC staining of human hepatocirrhosis using 16314-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 16314-1-AP (dGK antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse kidney tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16314-1-AP targets dGK in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen dGK fusion protein Ag9361
Full Name deoxyguanosine kinase
Calculated Molecular Weight 277 aa, 32 kDa
Observed Molecular Weight 28-32 kDa, 56-64 kDa
GenBank Accession NumberBC015757
Gene Symbol DGUOK
Gene ID (NCBI) 1716
RRIDAB_10638311
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Deoxyguanosine kinase (dGK) deficiency is a frequent cause of mitochondrial DNA depletion associated with a hepatocerebral phenotype. dGK is a member of the deoxyribonucleoside kinase family involved in phosphorylation of deoxyribonucleosides. In mammals, there are four deoxyribonucleoside kinases with overlapping specificities: two cytoplasmic enzymes, thymidine kinase 1 (TK1) and deoxycytidine kinase (dCK), and two mitochondrial enzymes, thymidine kinase 2 (TK2) and deoxyguanosine kinase (dGK). The human dGK protein is highly specific for purine substrates. Human dGK is a dimer of two identical subunits of 30 kDa. The three-dimensional structure of the human dGK monomer has been reported, providing an explanation for the substrate specificity of the enzyme. (PMID: 29786478, PMID: 11427893, PMID: 17073823)

Protocols

Product Specific Protocols
WB protocol for dGK antibody 16314-1-APDownload protocol
IHC protocol for dGK antibody 16314-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols